Slya Activates Fimb Expression and Type 1 Fimbriation in Escherichia Coli K-12*
نویسندگان
چکیده
1 School of Biosciences, University of Kent, Canterbury, CT2 7NJ, United Kingdom and 2 Institut de Biologie Physico-Chimique (Unité Propre de Recherche 9073, Centre National de la Recherche Scientifique), 13 Rue Pierre et Marrie Curie, 75005 Paris, France. Running title: SlyA and type 1 fimbriation Address correspondence to: Ian C. Blomfield, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, United Kingdom. Telephone +44 1227 823697; Fax +44 1227 763912; E-Mail: [email protected]
منابع مشابه
SlyA Protein Activates fimB Gene Expression and Type 1 Fimbriation in Escherichia coli K-12*
We have demonstrated that SlyA activates fimB expression and hence type 1 fimbriation, a virulence factor in Escherichia coli. SlyA is shown to bind to two operator sites (O(SA1) and O(SA2)), situated between 194 and 167 base pairs upstream of the fimB transcriptional start site. fimB expression is derepressed in an hns mutant and diminished by a slyA mutation in the presence of H-NS only. H-NS...
متن کاملLrp stimulates phase variation of type 1 fimbriation in Escherichia coli K-12.
The phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a short DNA element. This element (switch) acts in cis to control transcription of fimA, the major fimbrial subunit gene. Thus, fimA is transcribed when the switch is in one orientation (the on orientation) but not the other (the off orientation). The fim inversion requires either fimB (on-to-off o...
متن کاملRfaH Suppresses Small RNA MicA Inhibition of fimB Expression in Escherichia coli K-12
The phase variation (reversible on-off switching) of the type 1 fimbrial adhesin of Escherichia coli involves a DNA inversion catalyzed by FimB (switching in either direction) or FimE (on-to-off switching). Here, we demonstrate that RfaH activates expression of a FimB-LacZ protein fusion while having a modest inhibitory effect on a comparable fimB-lacZ operon construct and on a FimE-LacZ protei...
متن کاملHosA, a member of the SlyA family, regulates motility in enteropathogenic Escherichia coli.
In enteropathogenic and enterohemorraghic Escherichia coli (EPEC and EHEC), two members of the SlyA family of transcriptional regulators have been identified as SlyA. Western blot analysis of the wild type and the corresponding hosA and slyA deletion mutants indicated that SlyA and HosA are distinct proteins whose expression is not interdependent. Of 27 different E. coli strains (EPEC, EHEC, en...
متن کاملAlternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12
Haemolysin E is a cytolytic pore-forming toxin found in several Escherichia coli and Salmonella enterica strains. Expression of hlyE is repressed by the global regulator H-NS (histone-like nucleoid structuring protein), but can be activated by the regulator SlyA. Expression of a chromosomal hlyE-lacZ fusion in an E. coli slyA mutant was reduced to 60% of the wild-type level confirming a positiv...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011